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Accession IconE-MEXP-1135

Transcription profiling of gastric tissues from mice infected with H. pylori strain SS1 for 6 or 12 months

Organism Icon Mus musculus
Sample Icon 14 Downloadable Samples
Technology Badge Icon Affymetrix Mouse Expression 430A Array (moe430a)

Submitter Supplied Information

Description
12 C57BL/6 mice were infected orogastrically with the H. pylori strain SS1. After 6 and 12 months, 3 non-infected and 3 infected mice were sacrificed and stomachs isolated. Gastric tissues were disaggregated and total RNA were isolated by TRIzol extraction and then purified on RNeasy minicolumns. After synthesis of the first cDNA strand (In vitrogen), the double-stranded cDNA was obtained and used to produce biotin-labeled cRNA (Enzo Diagnostic). FRagmented cRNA was hybridized to GeneChip Mouse expression array 430A (Affymetrix).
PubMed ID
Total Samples
14
Submitter’s Institution
Source Repository

Samples

Show of 14 Total Samples
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Accession Code
Title
Sex
Age
Specimen part
Disease
Subject
Time
Processing Information
Additional Metadata
M12S1a
male
6.000
epithelial cell
active gastritis with low grade metaplasia
m12s1a
12
M12S1
male
6.000
epithelial cell
active gastritis with low grade metaplasia
m12s1
12
M6N4b
male
6.000
epithelial cell
normal
m6n4b
6
M6S5a
male
6.000
epithelial cell
active gastritis
m6s5a
6
M6S2
male
6.000
epithelial cell
active gastritis
m6s2
6
M12S3
male
6.000
epithelial cell
active gastritis with low grade metaplasia
m12s3
12
M12S4
male
6.000
epithelial cell
active gastritis with low grade metaplasia
m12s4
12
M6S1
male
6.000
epithelial cell
active gastritis
m6s1
6
M6N6
male
6.000
epithelial cell
normal
m6n6
6
M12N6
male
6.000
epithelial cell
normal
m12n6
12
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