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Accession IconE-MEXP-1407

Transcription profiling by array of S. cerevisiae wild type, SNF1, SNF4, and double knock outs to investigate SNF1/4 dependent gene expression

Organism Icon Saccharomyces cerevisiae
Sample Icon 12 Downloadable Samples
Technology Badge Icon Affymetrix Yeast Genome 2.0 Array (yeast2)

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Description
The protrotophic laboratory strain CEN.PK113-7D (MAT a) and three knock-out strains snf1, snf4 and snf1snf4 were grown in laboratory fermentors with a working volume of 1 litre at dilution rate (D) of 0.10 per hour (in triplicate for each strain). At steady state, samples from each of the 12 continuous cultures were taken and cooled below 2 degree C within ten seconds by mixing 40% sample and 60% crushed ice.
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12
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