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Accession IconGSE10345

Genome-wide analysis of transcriptional termination in E. coli

Organism Icon Escherichia coli
Sample Icon 20 Downloadable Samples
Technology Badge Icon Affymetrix E. coli Genome 2.0 Array (ecoli2)

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Transcription termination factor Rho is essential in enterobacteria. We inhibited Rho activity with bicyclomycin and used microarray experiments to assess Rho function on a genome-wide scale. Rho is a global regulator of gene expression that matches E. coli transcription to translational needs. Remarkably, genes that are most repressed by Rho are prophages and other horizontally-acquired portions of the genome. Elimination of these foreign DNA elements increases resistance to bicyclomycin. Although rho remains essential, such reduced-genome bacteria no longer require Rho cofactors NusA and NusG. Thus, Rho termination, supported by NusA and NusG, is required to suppress the toxic activity of foreign DNA.
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