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Accession IconGSE117981

Characterizing the gene expression profile of Prox1+ intestinal adenoma organoid cells

Organism Icon Mus musculus
Sample Icon 6 Downloadable Samples
Technology Badge IconIllumina MouseWG-6 v2.0 expression beadchip

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We isolated and selected intestinal adenoma organoids from Apcmin/+; Rosa26LSL-TdTomato; Prox1-CreERT2 mice. After the selection procedure without growth factors, we induced CreERT2 activity and the transcription of tdTomato to label Prox1+ cells by 300 nM 4-hydroxytamoxifen for 16h. tdTomato+ (Prox1+) and tdTomato- cells (enriched for Prox1- cells) were FACS sorted and total RNA was isolated.
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