Genetic ablation of either G6P-isomerase or dual LDHA/B in colon cancer cells LS174T , +/- MPP+ (1-methyl-4-phenylpyridinium)

In this study we explore the genomic re-wiring of a human colon cancer cell line (LS174T) in which fermentative glycolysis has been fully suppressed by CRISPR/Cas9 to disrupt glucose-6-phosphate isomerase (GPI-KO) or dual disruption of lactate dehydrogenases A and B (LDHAB-DKO). The effects of both KOs, had no effect on loss of viable cell function, corresponding to high concentrations of ATP production vs WT. All three clones were evaluated for transcriptomic changes using GeneChip™ Human Gene 2.1 ST Arrays by Affymetrix Inc assessing for mRNAs and long intergenic non-coding RNA transcripts.

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