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Accession IconSRP039386

Hepatocyte ductal metaplasia in chronic mouse liver injury

Organism Icon Mus musculus
Sample Icon 14 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
Expression profiling of hepatocytes-derived ductal cells with properties intermediate between mature hepatocytes and cholangiocytes Overall design: Chimeric adult mice were generated where mature hepatocytes were marked with a fluorescent red marker. Chronic injury was induced for ~6weeks and three cell types were isolated by FACS (Influx, BD) for expression analysis by RNAseq based on cell surface phenotype and origin: hepatocytes (n=3), hepatocyte-derived oval cells (1c3+, n=5), and cholangiocyte-derived oval cells (1c3+, n=5).
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14
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