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Accession IconSRP123295

Determining mRNA half-lives on a transcriptome-wide scale

Organism Icon Homo sapiens
Sample Icon 7 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Description
Translation and mRNA decay are intimately connected processes, and translational inhibition often precedes and stimulates transcript degradation. Here, we have focused on methods that allow determination of mRNA stability on a transcriptome-wide scale. We describe experimental and computational methods for the two most commonly used approaches (transcriptional inhibition and metabolic labeling), and we discuss associated caveats. Overall design: Metabolic labeling time courses (1, 2, 4, 8, 12, 24 hr) using 4SU were performed in HEK293.
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7
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