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Accession IconSRP125353

Investigate A2M treatment in liver of mice

Organism Icon Mus musculus
Sample Icon 11 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

Submitter Supplied Information

Description
Cancer resistance is a major cause for longevity of the naked mole-rat. Recent liver transcriptome analysis in this animal compared to wild-derived mice revealed higher expression of alpha2-macroglobulin (A2M) and cell adhesion molecules, which contribute to the naked mole-rat's cancer resistance. Notably, A2M is known to dramatically decrease with age in humans. We hypothesize that this might facilitate tumour development. Here we found that A2M modulates tumour cell adhesion, migration and growth by inhibition of tumour promoting signalling pathways, e.g. PI3K / AKT, SMAD and up-regulated PTEN via down-regulation of miR-21, in vitro and in tumour xenografts. A2M increases the expression of CD29 and CD44 but did not evoke EMT. Transcriptome analysis of A2M-treated tumour cells, xenografts and mouse liver demonstrated a multifaceted regulation of tumour promoting signalling pathways indicating a less tumorigenic environment mediated by A2M. By virtue of these multiple actions the naturally occurring A2M has strong potential as a novel therapeutic agent. Overall design: 11 samples: 5 treated with PBS, 6 treated with A2M
PubMed ID
Total Samples
11
Submitter’s Institution
No associated institution

Samples

Show of 11 Total Samples
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Title
Specimen part
Cell line
Treatment
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Processing Information
Additional Metadata
Liver_123
liver
cd1 nu/nu
pbs
liver
NA
Liver_126
liver
cd1 nu/nu
pbs
liver
NA
Liver_127
liver
cd1 nu/nu
pbs
liver
NA
Liver_128
liver
cd1 nu/nu
a2m
liver
NA
Liver_130
liver
cd1 nu/nu
a2m
liver
NA
Liver_131
liver
cd1 nu/nu
a2m
liver
NA
Liver_132
liver
cd1 nu/nu
a2m
liver
NA
Liver_124
liver
cd1 nu/nu
pbs
liver
NA
Liver_125
liver
cd1 nu/nu
pbs
liver
NA
Liver_129
liver
cd1 nu/nu
a2m
liver
NA
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