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Accession IconSRP165285

RNA-Seq of WT and constitutively methylated mESCs

Organism Icon Mus musculus
Sample Icon 12 Downloadable Samples
Technology Badge IconNextSeq 550

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Description
WT J1 and 3B3L cells (in which Dnmt3B and Dnm3L are constitutively expressed from an exogenous construct) were cultured under both serum/LIF and 2i/LIF conditions. 3B3L cells do not show ground state-associated hypomethylation phenotype. This experiment sought to analyse the gene expression changes between the two conditions. Overall design: Three biological replicates per condition J1 serum, J1 2i, 3B3-3l serum, 3B3-3l 2i.
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12
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