Filters
Technology
Platform
GSE86036
Homo sapiens
3 Downloadable Samples
Affymetrix Human Gene 2.1 ST Array (hugene21st)
Description
Leukemia Inhibitory Factor is an important cytokine of the IL family. Recent findings suggest it has a crucial role in cancer progression
Publication Title
Leukemia Inhibitory Factor Promotes Aggressiveness of Chordoma.
Sample Metadata Fields
Cell line
View Samples- Mus musculus
- 9 Downloadable Samples
Illumina HiSeq 2000
Description
Interleukin-15 (IL-15) and IL-2 possess distinct immunological functions despite both signaling through IL-2Rß and the common cytokine receptor ?-chain, ?c, We find that in the IL-15/IL-15Ra/IL-2Rß/?c quaternary complex structure, IL-15 heterodimerizes IL-2Rß and ?c identically to the IL-2/IL-2Ra/IL-2Rß/?c complex, despite differing receptor-binding chemistries. IL-15Ra dramatically increases the affinity of IL-15 for IL-2Rß, and this allostery is required for IL-15 trans-signaling versus IL-2 cis-signaling. Consistent with the identical IL-2Rß/?c dimer geometry, IL-2 and IL-15 exhibited similar signaling properties in lymphocytes, with any differences resulting from disparate receptor affinities. Thus, IL-15 and IL-2 induce similar signals, and the cytokine-specificity of IL-2Ra versus IL-15Ra determines cellular responsiveness. These results provide important new insights for specific development of IL-15- versus IL-2-based immunotherapeutics. Overall design: RNA-Seq is conducted in mouse CD8+ T cells, not treated or treated with IL2 or IL15 for indicated concentrations (1nM or 500nM) and times (4hr or 24hr).
Publication Title
Mechanistic and structural insight into the functional dichotomy between IL-2 and IL-15.
Sample Metadata Fields
Specimen part, Cell line, Treatment, Subject
View Samples- Rattus norvegicus
- 25 Downloadable Samples
- Affymetrix Rat Genome 230 2.0 Array (rat2302)
Description
The endoplasmic reticulum (ER) is the site of secretory lipoprotein production and de novo cholesterol synthesis, yet little is known about how these activities are coordinated with each other, or with the activity of the COPII machinery, which transports ER cargo to the Golgi. The Sar1B component of this machinery is mutated in Chylomicron Retention Disorder, establishing that this Sar1 isoform secures delivery of dietary lipids into the circulation.
Publication Title
The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis.
Sample Metadata Fields
No sample metadata fields
View Samples