Analysis of gene expression change of normal cells, human fore skin fibroblast (HFFs) upon over-expression of c-Myc
Oncogene-induced senescence mediated by c-Myc requires USP10 dependent deubiquitination and stabilization of p14ARF.
Cell line
View SamplesAnalysis of miRNA-targeted cellular NMD substrates in HeLa cell. The hypothesis tested in the present study was that endogenous NMD substrates containing long 3' untranslated region may targeted for miRNA. Results provide important information expanding the roles of miRISC in the posttranscriptional regulation of gene expression: a new cross-talk between miRNA-mediated gene silencing and NMD.
microRNA/Argonaute 2 regulates nonsense-mediated messenger RNA decay.
No sample metadata fields
View Sampleshuman Transcriptomehuman Transcriptomehuman Transcriptomehuman Transcriptomehuman Transcriptome
No associated publication
Sex, Age, Specimen part
View SamplesThis study revealed the EP impact on gene expression in fruit fly and provided new insight into the mechanisms of this response, which is helpful for understanding EP toxicity to humans.
No associated publication
Sex, Specimen part
View SamplesTranscriptomic sequencing of human gastric cancer cell line (AGS) upon citral treatment
No associated publication
Sex, Specimen part, Disease, Cell line
View SamplesIt has been reported that IRE1-bZIP60 pathway is conserved for the signal transduction and gene regulation in response to ER stress including high temperature among human, yeast, Arabidopsis, rice and maize. However, the information of their counterparts in wheat is limited and, especially, the biological relevance of the equivalent pathway contributing to heat tolerance is still ambiguous. In present study, we identified heat stress-induced wheat bZIP60 and investigated its splicing pattern depending on IRE1.
No associated publication
Specimen part, Treatment
View SamplesIn this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 ?C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 ?C under dark conditions, the immature embryos were collected, and stored at -70 ?C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
No associated publication
Specimen part
View SamplesIn this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
No associated publication
Specimen part
View SamplesIn this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
No associated publication
Specimen part
View SamplesIn this study, the immature embyros of inbred line Z31and hybrid HiII were heated in 45 C water bath for 2 min, and then infected with Agrobacterium suspension for 5 min. After infection, the immature embryos were transferred onto CO-medium with or without 100 mg L-1 cysteine which is freshly prepared and filter-sterilized. After culturing for 3 days at 25 C under dark conditions, the immature embryos were collected, and stored at -70 C for RNA extraction. The genes up-regulated and down regulated both in Z31 and HiII were analyzed, and elucidate the role of cysteine in improving the efficiency of Agrobacterium infection of maize immature embryo, and provide important information for the improvement of the maize transformation.
No associated publication
Specimen part
View Samples