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GSE9316
Mus musculus
12 Downloadable Samples
Affymetrix Mouse Genome 430 2.0 Array (mouse4302)
Description
Th17 cells are enriched by sorting FR4-CD4+ T cells from SKG mice. A large number of Th17 cells also develop spontaneously when CD4+ T cells from IFN-g-deficient (IFN-g-/-) BALB/c mice are transferred to T cell-deficient RAG2-deficient (RAG2-/-) mice and subjected to homeostatic proliferation, whereas they fail to develop in similar transfer of IL-6-deficient (IL-6-/-) CD4+ T cells to IL-6-/- RAG2-/- mice.
Publication Title
Preferential recruitment of CCR6-expressing Th17 cells to inflamed joints via CCL20 in rheumatoid arthritis and its animal model.
Sample Metadata Fields
Sex, Age, Specimen part
View Samples- Homo sapiens
- 6 Downloadable Samples
- Affymetrix Human Gene 1.0 ST Array (hugene10st)
Description
Comparison of gene expressions among osteogenic differentiated cells with retinoic acid, those without retinoic acid and cells before induction
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Mus musculus
- 4 Downloadable Samples
Illumina HiSeq 3000
Description
Regnase-1 and Roquin are RNA binding proteins essential for degradation of inflammatory mRNAs and the maintenance of immune homeostasis. Recent researches have showed that Regnase-1 and Roquin target overlapping sets of mRNAs with common stem-loop structures but localize in different structures within the cell, controlling immune-related RNAs in distinct spatiotemporal processes. Regnase-1 and Roquin are expressed in T cells, and mutations of both Regnase-1 and Roquin in T cells leads to massive lymphocyte activation. Furthermore, mutation of both Regnase-1 and Roquin leads to a huge increase in the Th1, but not Th2 or Th17 population in spleens compared to T cells with either a single Regnase-1 or Roquin deficiency. To investigate Regnase-1- and Roquin-regulated genes, transcriptome analysis was conducted using CD4 T cells lacking Regnase-1 and Roquin.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 3 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
To identify transcriptio factors responsible for CXCL13 production by human CD4+ T cells, we differentiated CXCL13-producing CD4+ T cells in vitro under TGF--positive inflammatory conditions and conducted transcriptome analysis.
Publication Title
Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments.
Sample Metadata Fields
Specimen part
View Samples- Mus musculus
- 8 Downloadable Samples
- Illumina HiSeq 2500
Description
Gene expression in response to inflammatory stimuli is controlled by both transcriptional and posttranscriptional mechanisms in immune cells. Posttranscriptional regulation that modifies mRNA stability and translation provides rapid and flexible control of gene expression and control of mRNAs stability is important for coordinating the initiation and resolution of inflammation. However, the posttranscriptional mechanisms in innate immunity remain to be clarified. This study is aiming to investigate the posttranscriptional mechanisms in innate immunity based on the roles of RNA binding proteins and RNase we identified.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Mus musculus
- 4 Downloadable Samples
- Illumina HiSeq 2500
Description
Duodenums were isolated from Regnase-1-deficient mice and their controls. Tissues were immediately homogenized in TRIzol (Life Technologies). Total RNA was isolated according to the manufacture''s protocol.
Publication Title
No associated publication
Sample Metadata Fields
Age, Specimen part, Cell line
View Samples- Mus musculus
- 5 Downloadable Samples
- Affymetrix Mouse Gene 2.0 ST Array (mogene20st)
Description
Sensorineural hearing loss (SHL) is a relatively common disease, and studies have suggested viral infection as a major cause. In the inner ear, the blood-labyrinthine barrier prevents access of the peripheral immune system; therefore, the immune system remains poorly understood. Here we found that cochlear accessory supporting cells (SCs), which are anchored by tight junctions, are organized tissue-resident macrophages. Virus-infected supporting cells change into activated macrophages and protect audiosensory receptor hair cells (HCs) against virus infection by producing interferon (IFN)-/. Moreover, we also observed bacterial phagocytosis by SCs. However, tumour necrosis factor-related apoptosis-inducing ligand (Trail), produced by virus-infected SCs, induced sensory hair loss and HC death by necroptosis. Notably, corticosteroid, the only effective drug for SHL, inhibited the virus-induced macrophage change of SCs. These results revealed an inner ear immune system, and suggest a possible mechanism for virus-induced SHL.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Homo sapiens
- 750 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
Mesenchymal stem cells (MSCs) are an essential component of the bone marrow (BM) microenvironment and have shown to support cancer evolution in multiple myeloma (MM). Despite the increasing evidence that MM MSCs differ from their healthy counterparts, little knowledge exists as to whether MSCs independently influence disease outcome. The aim of the present study was to determine the importance of MSCs in disease progression and outcome in MM.
Publication Title
The Pattern of Mesenchymal Stem Cell Expression Is an Independent Marker of Outcome in Multiple Myeloma.
Sample Metadata Fields
Specimen part, Disease, Subject
View Samples- Homo sapiens
- 374 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
Obesity has considerable effects on morbidity and mortality, and the prevalence of obesity has been increasing rapidly worldwide during the past two decades. Even if obesity affects the entire individual, adipose tissue plays a central role in the development of obesity. Expression profiling of adipose tissue may give insights into the mechanisms contributing to obesity and obesity-related disorders.
Publication Title
Adipose tissue resting energy expenditure and expression of genes involved in mitochondrial function are higher in women than in men.
Sample Metadata Fields
Sex, Specimen part
View Samples- Homo sapiens
- 130 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
Purpose: Because dexamethasone remains a key component of myeloma therapy, we wished to examine the correlation of baseline and relapse expression levels of the glucocorticoid receptor gene NR3C1 with other clinical features. Experimental Design: We investigated the clinical impact of gene expression profiling (GEP)derived expression levels of NR3C1 in 351 patients with GEP data available at baseline and in 130 with data available at relapse, among 668 subjects accrued to Total Therapy 2 (TT2).
Publication Title
Thalidomide in total therapy 2 overcomes inferior prognosis of myeloma with low expression of the glucocorticoid receptor gene NR3C1.
Sample Metadata Fields
Disease, Treatment
View Samples