Filters
Technology
Platform
Drosophila melanogaster
12 Downloadable Samples
Illumina HiSeq 2000, Illumina HiSeq 2500
Description
Spiroplasma (Mollicutes) is one of the heritable bacterial endosymbionts of Drosophila species. Several strains like S. poulsonii manipulate host reproduction in a selfish manner. When females of D. melanogaster are infected with natural S. poulsonii strain MSRO (melanogaster sex ratio organism), only male offspring are killed during embryogenesis, and this phenomenon is called male-killing. To understand the molecular mechanism of male-killing, we compared gene expression profiles between MSRO-infected and uninfected embryos of D. melanogaster by using RNA-sequencing (RNA-seq). For embryonic sexing, we employed a transgenic reporter strain Sex-lethal (Sxl)-Pe-EGFP, which expresses GFP only in females. We collected female and male embryos at stage 10-11, when abnormal apoptosis associated with male-killing starts to occur in male progenies. For each sample, we analyzed three biological replicates.
Publication Title
Male-killing symbiont damages host's dosage-compensated sex chromosome to induce embryonic apoptosis.
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 16 Downloadable Samples
- Illumina HiSeq 4000
Description
RNA was isolated from colorectal cancer (HCT116) and normal colon cells (HCoEpic) treated with toxic agents (bisphenol A (BPA), hexabromocyclododecane (HBCD), 4-tert-octylphenol (OP)) or Vehicle (DMSO) and then preformed NGS using Illumina protocol.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Disease, Cell line, Treatment
View Samples- Mus musculus
- 6 Downloadable Samples
- Illumina HiSeq 2000
Description
Tmem67 knock-out mouse model of Meckel-Gruber syndrome type 3 (MKS3, MIM #607361) and Joubert syndrome type (JBTS6, #610688): investigation into differential gene expression in the early post-natal (P0) cerebullum
Publication Title
Variable expressivity of ciliopathy neurological phenotypes that encompass Meckel-Gruber syndrome and Joubert syndrome is caused by complex de-regulated ciliogenesis, Shh and Wnt signalling defects.
Sample Metadata Fields
No sample metadata fields
View Samples- Saccharomyces cerevisiae
- 6 Downloadable Samples
Affymetrix Yeast Genome 2.0 Array (yeast2)
Description
We systematically investigated the impact of genetic variation and the environment on protein overproduction costs in Saccharomyces cerevisiae
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Drosophila melanogaster
- 6 Downloadable Samples
- Illumina HiSeq 2000
Description
Early embryo RNA-seq sequencing
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples
SRP090913- Arabidopsis thaliana
- 12 Downloadable Samples
- Illumina HiSeq 3000
Description
Cell wall modifications in response to low temperature compensation
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Homo sapiens
- 4 Downloadable Samples
- Illumina HiSeq 2000
Description
In this study, we purified periodontal ligament cell clones committed to osteoblastic/cementoblastic phenotype (C-O clones) and clones committed to fibroblastic phenotype (C-F clones), and employed RNA-seq to describe the differential transcriptional profile of osteoblastic/cementoblastic and fibroblastic cell clones from human periodontal ligament. The understanding of differences in periodontal ligament subpopulations can help to elucitade the favorable to formation of mineralizing and non-mineralizing tissues of periodontium.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part
View Samples- Homo sapiens
- 4 Downloadable Samples
- Illumina HiSeq 2000
Description
In this study, we purified periodontal ligament cell clones committed to osteoblastic/cementoblastic phenotype (C-O clones) and clones committed to fibroblastic phenotype (C-F clones), and employed RNA-seq to describe the differential transcriptional profile of osteoblastic/cementoblastic and fibroblastic cell clones from human periodontal ligament. The understanding of differences in periodontal ligament subpopulations can help to elucitade the favorable to formation of mineralizing and non-mineralizing tissues of periodontium.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Treatment
View Samples SRP050879- Danio rerio
- 1 Downloadable Sample
- IlluminaHiSeq2000
Description
we aimed to calculate the expression level of some special genes
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 27 Downloadable Samples
- Affymetrix Mouse Genome 430 2.0 Array (mouse4302)
Description
We examined the effect of quercetin on the gene expression and function of epididymal adipose tissue (EAT) in Western diet-induced obese mice. Quercetin suppressed the increase in the number of macrophages and the decrease in the ratio of CD4+ to CD8+ T cells in EAT, and the elevation of plasma leptin and TNF levels in mice fed the Western diet. Comprehensive gene expression analysis revealed that quercetin suppressed gene expression associated with the accumulation and activation of immune cells, including macrophages and lymphocytes in EAT. It also improved the expression of the oxidative stress-sensitive transcription factor NFB, NADPH oxidases, and antioxidant enzymes. Quercetin markedly increased gene expression associated with mitochondrial oxidative phosphorylation and mitochondrial DNA Quercetin most likely universally suppresses the accumulation and activation of immune cells, including anti-inflammatory cells, whereas it specifically increased gene expression associated with mitochondrial oxidative phosphorylation. Suppression of oxidative stress and NFB activity likely contributed to the prevention of the accumulation and activation of immune cells and resulting chronic inflammation.
Publication Title
Quercetin suppresses immune cell accumulation and improves mitochondrial gene expression in adipose tissue of diet-induced obese mice.
Sample Metadata Fields
Sex, Specimen part
View Samples